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. 2016 Jul 25;113(32):9069–9074. doi: 10.1073/pnas.1609707113

Fig. 1.

Fig. 1.

VIDD is associated with defective RyR1 in human diaphragm muscle. Representative immunoblots (A) of immunoprecipitated RyR1 from human diaphragm samples collected after short-term (control) and long-term (MV) controlled mechanical ventilation in humans (Table S1). (Each blot corresponds to adjacent wells of the same gel.) Bar graphs (B) show quantification of immunoblots, relative to total RyR1 (mean ± SEM, n = 10 and 9 in control and MV, respectively, *P < 0.05, MV vs. control). CysNO, thio-nitrosylation; DNP, 2,4-dinitrophenylhydrazone; P−RyR1, phosphorylated RyR1 (at serine 2844). (C) Single-channel traces of RyR1 incorporated in planar lipid bilayers with 150 nM Ca2+ in the cis chamber, corresponding to representative experiments performed with human diaphragm biopsies from short-term and long-term MV groups. (D) Controlled mechanical ventilation increases RyR1 PO: Mean PO was 0.0006 ± 0.0003 in control (n = 18), and after MV, the PO increased to 0.0167 ± 0.00754 (n = 40).