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ProSAAS internalization. SH-SY5Y cells were incubated in the presence of recombinant proSAAS 1–180 (1 µM) or ovalbumin (1 µM) continuously for 18 h. Cells were then washed, fixed, permabilized, and stained for proSAAS (red) using antiserum LS45 IgG (1:1,000) and DAPI (white). (Scale bar: 30 μm.)
