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Results at intermediate steps in CPD-seq procedure. Yeast cells were irradiated with 125 J/m2 UVC light. Samples were taken before UV treatment (“No”), immediately after UV (0 h), and after 1 h of repair (1 h). (A) Isolated genomic DNA. (B) Sonicated genomic DNA. (C) PCR confirmation of trP1 adaptor ligation. (D) PCR confirmation of 3′OH blocking by TdT and ddATP (see SI Materials and Methods). (E) Gel verification of CPD-seq libraries.
