Fig. S5.

(A) Characterization of dopaminergic neurons derived from healthy control iPS cells. (A, i) Embryoid bodies (EB) were differentiated to neural precursor cells (PREC) and then manually passaged and matured to neurons (NEUR), as described in SI Materials and Methods. (A, ii and iii) Immunostaining for dopaminergic (TH and GIRK2) (ii) and neuronal (β-III tubulin) markers (iii). (Scale bars, 10 µm.) (B) The expression of neuronal markers (TH, Tau, and α-Syn) at the appropriate stage of differentiation was confirmed by immunoblotting.