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(A) The rough eye phenotype of A53T α-synuclein was not rescued in flies coexpressing in the eye RNAi to knock down AMSH, Usp14, or Usp47. (B) The rough eye phenotype of pathogenic expanded huntingtin (Htt) was not rescued in the corresponding double-transgenic line expressing RNAi against Usp8 (Htt/Usp8 KD). (C) The functional efficiency of Usp8 knockdown was confirmed by detecting the previously documented wing defect when the RNAi was expressed under the MS1096-GAL4 driver.
