Figure 2.

HPLC analysis of BaP metabolites formed by human recombinant P450 1A1 expressed in Supersomes in the presence of NADPH (A). Structures of BaP metabolites formed by human P450 1A1 (B). Amounts of BaP metabolites formed by human recombinant P450 1A1 expressed in Supersomes in the presence of either NADPH or NADH and the effect of cytochrome b₅ (cyt b₅) on this metabolism (C). Note that besides human P450 1A1 and POR overexpressed in Supersomes, this enzymatic system also contained mEH and CBR. Values represent the mean ± SD from three parallel measurements. ***P < 0.001 (Student’s t-test), significantly different from incubations using NADPH as cofactor; ^ΔΔΔP < 0.001 (Student’s t-test), significantly different from incubations without cytochrome b₅.