Spatial expression pattern of ddcp1 encoding a Duf domain protein. (A–C) ddcp1 was expressed along the edge of neural plate. Notably, two stronger staining patches (arrows) were detected in whole mount embryos (A) and transverse sections (B). With the closure of neural tube, the two signaling patches merged and were located at the frontal region of the head (D–G). Ant, anterior view; lat, lateral view. Ac, archerteron; ev, eye vesicle; bv, brain vesicle; hb, hindbrain.