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. Author manuscript; available in PMC: 2017 Mar 1.
Published in final edited form as: Obesity (Silver Spring). 2016 Feb 1;24(3):678–686. doi: 10.1002/oby.21425

Figure 1. Resistin-mediated increase in SGK1 expression is dose-dependent.

Figure 1

A: mRNA. HAoSMC were serum-starved for 48h and stimulated with increasing doses of resistin (0–100ng/ml) for 1h and SGK1 mRNA was quantified by qRT-PCR. n=3. B: Representative western blot image of protein extracted from HAoSMC serum-starved for 48h and stimulated with increasing doses of resistin (0–100ng/ml) for 1h C: SGK1 protein levels were normalized to GAPDH and quantified using NIH Image J. Data were analyzed by One-Way ANOVA followed by appropriate post hoc test for multiple comparisons and expressed as ± SEM, *P<0.05 vs 0ng/ml control. (n=3 independent studies).