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. Author manuscript; available in PMC: 2017 Mar 1.
Published in final edited form as: Obesity (Silver Spring). 2016 Feb 1;24(3):678–686. doi: 10.1002/oby.21425

Figure 3. Resistin-mediated SGK1 phosphorylation is PI3K-dependent.

Figure 3

A: HAoSMC were pre-treated with 50µM LY294002 or vehicle for 1h. Media was replaced with fresh LY294002 and incubated in the presence or absence of 40ng/ml resistin for 3h. SGK1 phosphorylation at Ser422 was examined by western blot. B: phospho-SGK1 protein levels were normalized to total SGK1 and quantified using NIH Image J. Data were analyzed by One-Way ANOVA followed by appropriate post hoc test for multiple comparisons and expressed as ± SEM *P< 0.05 vs Vehicle; *P< 0.05 vs Resistin, *P<0.05. (n=4 independent studies).