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. Author manuscript; available in PMC: 2017 Mar 1.
Published in final edited form as: Obesity (Silver Spring). 2016 Feb 1;24(3):678–686. doi: 10.1002/oby.21425

Figure 4. Resistin-mediated SGK1 phosphorylation is TLR4-dependent.

Figure 4

A: PCR product verification on agarose gel of siRNA TLR4 knockdown. B: mRNA quantification of HAoSMC transfected with scramble or siRNA TLR4. C: Representative western blot image of phospho- and total SGK1 protein levels from HAoSMC transfected with scramble or siRNA TLR4 in the presence or absence of 40ng/ml resistin. D: Quantitative analysis of phospho- and total SGK1 were performed with NIH Image J. Data were analyzed by Student’s t-test or Two-Way ANOVA followed by appropriate post hoc test for multiple comparisons and expressed as ± SEM *P<0.01 vs scramble control; **P<0.05 vs scramble resistin. (n=3–5 independent studies).