Table 3.

qPCR primer sequences and characteristics

Gene	Accesion no.	Primer source	Primer sequence (5′ → 3′)	Orientation	Tm (°C)	Amplicon lenght (bp)	References
TNF-α	NM_214022	Pig	ACTGCACTTCGAGGTTATCGG	forward	60	118	[54]
			GGCGACGGGCTTATCTGA	reverse	60
IL-8	NM_213867.1	Pig	GCTCTCTGTGAGGCTGCAGTTC	forward	58	79	[54]
			AAGGTGTGGAATGCGTATTTATGC	reverse	54
IL-6	NM_214399	Pig	GGCAAAAGGGAAAGAATCCAG	forward	57	87	[54]
			CGTTCTGTGACTGCAGCTTATCC	reverse	61
IL-1β	NM_214055	Pig	ATGCTGAAGGCTCTCCACCTC	forward	62	89	[55]
			TTGTTGCTATCATCTCCTTGCAC	reverse	59
IL-10	NM_214041.1	Pig	GGCCCAGTGAAGAGTTTCTTTC	forward	54	51	[54]
			CAACAAGTCGCCCATCTGGT	reverse	55
NFkB1	NM_001048232.1	Pig	TCGCTGCCAAAGAAGGACAT	forward	54	101	[55]
			AGCGTTCAGACCTTCACCGT
GAPDH	NM_001206359.1	Pig	ACTCACTCTTCTACCTTTGATGCT	forward	49	100	[54]
			TGTTGCTGTAGCCAAATTCA	reverse	56
B-actina	NM_213978.1	Pig	GGACTTCGAGCAGGAGATGG	forward	60	230	[56]
			GCACCGTGTTTGCGTAGAGG	reverse	62