Table 2.
List of genes with complete deletion in the isoniazid-resistant outbreak strains
| Genes name (function) | Isolates: 02.292, 03.039, 04.018, 04.211, 04.493, 04.503, 07.116 | 03.313 | 05.177 Control |
|---|---|---|---|
| Phirv1 phage proteins (part of genomic island) | ✘ | ✘ | ✓ |
| Rv1673c (conserved hypothetical protein) | ✘ | ✘ | ✓ |
| Rv1675c (probable cAMP and macrophage regulator) | ✘ | ✘ | ✓ |
| plcD (phosphoesterase) | ✘ | ✘ | ✓ |
| Wag22 (antigen member, PE family) | ✘ | ✘ | ✓ |
| Rv1760 (possible triacylglycerol synthase) | ✘ | ✘ | ✓ |
| Rv1761c (unknown protein) | ✘ | ✘ | ✓ |
| Rv1762c (unknown protein) | ✘ | ✘ | ✓ |
| plcB (phosphoesterase) | ✘ | ✓ | ✓ |
| plcA (membrane associated phospholipase) | ✘ | ✓ | ✓ |
| PPE54 (part of PPE family) | ✘ | ✓ | ✓ |
| PE_PGRS50 (antigen member, PE family) | ✘ | ✘ | ✓ |
| PPE55 (part of PPE family) | ✘ | ✘ | ✓ |
| Rv3349c (probable transposase) | ✘ | ✘ | ✓ |
| Rv3371 (possible triacylglycerol synthase) | ✘ | ✘ | ✓ |
| Rv3486 (membrane protein, function unknown) | ✘ | ✘ | ✓ |
Comparison is also made with possible outbreak strain 03.313 (phylogenetically related but only streptomycin monoresistant). The symbol ✘ indicates that the gene contains extensive deletions, while the symbol ✓indicates that the gene is still present. Deletions were found in 16 genes in the isoniazid-resistant tuberculosis outbreak isolates compared with the control strain 05.177. Additional comparison with another possible outbreak strain (03.313) reduced the total common deletions to 13 genes. PE and PPE: proline-glutamate (PE) and proline-proline-glutamate (PPE)