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. 2016 Aug 17;7:117. doi: 10.1186/s13287-016-0378-0

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Proliferation capacity. a Macroscopic and microscopic view of colony-forming units (CFUs) determined by crystal violet staining. The number of viable ASC colonies formed was significantly more abundant in the N groups than in the 6R group. b Quantitative analysis of CFUs. CFU formation in the 6 N group was 2.5-fold higher than in the 6R group (^* p < 0.05). c Cell Counting Kit-8 (CCK-8) assay with 10 % fetal bovine serum (FBS). The cell numbers in the 6R group were significantly lower than numbers in the N group after day 7 (^** p < 0.01). d CCK-8 assay with 1 % FBS to analyze cellular growth under stress conditions. The cell numbers in the 6R group were higher than in the other groups throughout the entire experimental period. However, statistically significant differences were not obtained