Fig. 2.

miR-15b mimic and inhibitor delivery into LYO and MYO cells via transient transfection. a Relative miR-15b expression in miR-15b mimic transfected cells 24 h post transfection. b Relative expression level of miR-15b in cells 48 h after miR-15b mimic transfection. c Influence of miR-15b inhibitor on miR-15b expression in cells 24 h after transfection. d Relative miR-15b expression in miR-15b inhibitor transfected cells 48 h post transfection. Cells without miR-15b mimic or miR-15b inhibitor transfection were used as control group, which was abbreviated as cell blank. Quantitative RT-PCR experiment was performed as described in methods. The data were presented as mean ± SD and analyzed using Student’s t-test (two tail, unequal variance) and Mann–Whitney U test appropriately. The experiments were conducted in triplicate. Statistical significance was noted as *** p < 0.001