Fig. 3.

Inhibition of RECK mRNA expression by transfection miR-15b mimic in different type of cells. a qRT-PCR demonstrating decreased expression of RECK mRNA in cpLYO and cpMYO cells at 24 h post transfection with miR-15b mimic. b Reduction of RECK mRNA observed in miRNA-15b mimic transfected cpLYO, cpMYO and pLYO cells 48 h post transfection. Cells without miR-15b mimic transfection were used as control group, which was abbreviated as cell blank. Quantitative RT-PCR assay was performed as described in methods section. All data were presented as mean ± SD and analyzed using Student’s t-test (two tail, unequal variance) and Mann–Whitney U test when applicable. The experiments were performed in triplicate. Statistical significance was noted as* p < 0.05, ** p < 0.005, *** p < 0.001