Fig. 5.

miR-15b is predicted to bind to the 3’-UTR of RECK and regulates RECK expression. a Scheme of the potential binding sites of miR-15b in the 3’-UTR of RECK. RECK mRNA has the potential complimentary binding site with miR-15b within its 3’-UTR. b miR-15b regulation of RECK protein expression in cpLYO cells. Proteins from cpLYO cells transfected with either miR-15b mimic or inhibitor at 10 nM. After 48 h, the lysates were analyzed by Western blot. c miR-15b regulation of RECK protein expression in cpMYO cells. d miR-15b regulation of RECK protein expression in pLYO cells. e miR-15b regulation of RECK protein expression in pMYO cells. Data were presented as means ± SD. Statistical comparisons between miR-15b mimic/inhibitor transfected cells and mimic/inhibitor negative control cells was analyzed using Student’s t-test (two tail, unequal variance) and Mann–Whitney U test appropriately. Statistical significance was noted as *p < 0.05, **p < 0.005, ***p < 0.001