Abstract
Neutrophils from diabetic patients controlled with insulin showed impaired phagocytosis of a yeast, Candida guilliermondii. The defect was detected by measuring the initial rate of phagocytosis at optimal concentrations of phagocytes and organisms in autologous plasma. By mixing normal neutrophils in diabetic plasma and vice versa both cellular and plasma abnormalities were shown. The defect was reproduced by incubating normal plasma at a D-glucose concentration of 15 mmol/l for 3 h or for shorter periods at higher concentrations of glucose. The data suggest that defective phagocytosis is partly due to a reaction between glucose and the plasma proteins concerned with opsonisation. Defective phagocytosis may be important not only in coping with infections but also in other diabetic complications as plasma proteins are concerned with the removal of damaged or effete cells as well as foreign antigens.
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Selected References
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- BYBEE J. D., ROGERS D. E. THE PHAGOCYTIC ACTIVITY OF POLYMORPHONUCLEAR LEUKOCYTES OBTAINED FROM PATIENTS WITH DIABETES MELLITUS. J Lab Clin Med. 1964 Jul;64:1–13. [PubMed] [Google Scholar]
- Bagdade J. D., Nielson K. L., Bulger R. J. Reversible abnormalities in phagocytic function in poorly controlled diabetic patients. Am J Med Sci. 1972 Jun;263(6):451–456. doi: 10.1097/00000441-197206000-00005. [DOI] [PubMed] [Google Scholar]
- Bagdade J. D., Root R. K., Bulger R. J. Impaired leukocyte function in patients with poorly controlled diabetes. Diabetes. 1974 Jan;23(1):9–15. doi: 10.2337/diab.23.1.9. [DOI] [PubMed] [Google Scholar]
- Bagdade J. D., Stewart M., Walters E. Impaired granulocyte adherence. A reversible defect in host defense in patients with poorly controlled diabetes. Diabetes. 1978 Jun;27(6):677–681. doi: 10.2337/diab.27.6.677. [DOI] [PubMed] [Google Scholar]
- Crosby B., Allison F., Jr Phagocytic and bactericidal capacity of polymorphonuclear leucocytes recovered from venous blood of human beings. Proc Soc Exp Biol Med. 1966 Dec;123(3):660–664. doi: 10.3181/00379727-123-31570. [DOI] [PubMed] [Google Scholar]
- El-Maalem H., Fletcher J. Defective neutrophil function in chronic granulocytic leukaemia. Br J Haematol. 1976 Sep;34(1):95–103. doi: 10.1111/j.1365-2141.1976.tb00178.x. [DOI] [PubMed] [Google Scholar]
- Kay M. M. Mechanism of removal of senescent cells by human macrophages in situ. Proc Natl Acad Sci U S A. 1975 Sep;72(9):3521–3525. doi: 10.1073/pnas.72.9.3521. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Miller M. E., Baker L. Leukocyte functions in juvenile diabetes mellitus: humoral and cellular aspects. J Pediatr. 1972 Nov;81(5):979–982. doi: 10.1016/s0022-3476(72)80555-9. [DOI] [PubMed] [Google Scholar]
- Molenaar D. M., Palumbo P. J., Wilson W. R., Ritts R. E., Jr Leukocyte chemotaxis in diabetic patients and their nondiabetic first-degree relatives. Diabetes. 1976;25(2 Suppl):880–883. [PubMed] [Google Scholar]
- Munro J. F. Diabetes and infection. J Antimicrob Chemother. 1976 Dec;2(4):322–324. doi: 10.1093/jac/2.4.322. [DOI] [PubMed] [Google Scholar]
- Nolan C. M., Beaty H. N., Bagdade J. D. Further characterization of the impaired bactericidal function of granulocytes in patients with poorly controlled diabetes. Diabetes. 1978 Sep;27(9):889–894. doi: 10.2337/diab.27.9.889. [DOI] [PubMed] [Google Scholar]
- Scribner D. J., Fahrney D. Neutrophil receptors for IgG and complement: their roles in the attachment and ingestion phases of phagocytosis. J Immunol. 1976 Apr;116(4):892–897. [PubMed] [Google Scholar]
- Tan J. S., Anderson J. L., Watanakunakorn C., Phair J. P. Neutrophil dysfunction in diabetes mellitus. J Lab Clin Med. 1975 Jan;85(1):26–33. [PubMed] [Google Scholar]