FIG 3.
APOBEC3 does not protect mice from acute or latent MHV68 infection. (A) MHV68 (103 PFU) was intranasally introduced into each genotype of mice as indicated. The mice were sacrificed, and lung tissue was collected 7 dpi. Whole lung tissues were homogenized in 1 ml culture medium, and the supernatants of homogenates were 10-fold serially diluted. The titer of each dilution was determined on NIH3T12 cells, and they were stained and fixed at 5 dpi. Plaques were counted, and the viral titers are indicated as mean PFU/ml (n = 6 for C57BL/6 and A3KO, 5 for A3Ahigh and A3Ghigh, and 4 for Tmem173). (B and C) MHV68 (103 PFU) was intranasally injected into mice of each genotype as indicated. The mice were sacrificed, and the lung (B) and spleen (C) tissues were collected 16 days after injection. DNA was isolated and subjected to PCR with primers specific to ORF12. Values are indicated as MHV68 copy numbers normalized to mouse GAPDH (n = 5, 5, 4, 5, and 4 for WT, A3KO, A3Ahigh, A3Ghigh, and Tmem, respectively). NS, not significantly different by either two-way unpaired t test or one-way ANOVA.