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. 2016 Aug 12;90(17):7628–7639. doi: 10.1128/JVI.00746-16

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Copyright © 2016 Mullapudi et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 4 — Impact of disruption of the Cys87-Cys89 disulfide bond on stability and infectivity of SAFV-3. (A) A Sybr green fluorescence assay was performed to measure the stability of SAFV-3 particles. SAFV-3 virions were mixed with Sybr green dye II and heated to the indicated temperatures (x axis). The fluorescence signal increases as the dye binds to RNA that is released from thermally destabilized particles. The black line represents native SAFV-3 virions, and the red line represents SAFV-3 treated with 10 mM DTT. Error bars indicate the standard deviations of the measurements. See Materials and Methods for additional details. (B) Effect of DTT treatment on infectivity of SAFV-3 and EV71. Fractions of dead cells were determined based on propidium iodide staining.