FIG 5.

Release of SAFV-3 genome results in the formation of empty particles that disintegrate into pentamers. (A) Cryo-EM images of native virions. (B) SAFV-3 particles heated to 42°C for 2 min. The sample contained A particles and a few empty capsids. (C) Pentamers of capsid protein protomers prepared by incubating SAFV-3 virions at 42°C for 2 min and at 23°C for 5 min. The inset in panel C shows top and side view projections of pentamers of SAFV-3 protomers calculated from the structure of capsid proteins.