FIG 1.

In vitro neutralization by combinations of CD4bs antibodies with CD4i antibodies. (A) HIV-1 isolates 89.6, ADA, and SG3 were preincubated for 1 h with a fixed total concentration (0.3 μg/ml for 89.6 and ADA and 0.005 μg/ml for SG3) but various ratios of VRC01-E51 mixtures. TZM-bl cells were then added and incubated for 48 h. Infection was measured by the percent luciferase expression in the absence of inhibitor. (B) Experiment similar to that for panel A except that the indicated isolates were preincubated for 1 h with a fixed total concentration (0.3 μg/ml for 89.6 and ADA and 1.0 μg/ml for SA32) but various ratios of VRC01-412d mixtures. Error bars represent standard error of mean (SEM).