FIG 2.

Single-color immunofluorescence detects both rAAV8 and HSV-1 in mouse DRG. (A and B) Mice were infected with ssAAV-GFP only. DRG were harvested 14 dpi. Tissue sections were incubated with anti-GFP primary antibody (A) or with no primary antibody (B). Primary antibody was detected by the use of FITC-labeled secondary antibody. (C and D) Mice were infected with KOS/62 only. DRG were harvested 4 dpi. Tissue sections were incubated with anti-β-Gal primary antibody (C) or with no primary antibody (D). Primary antibody was detected by Texas Red-labeled secondary antibody.