FIG 6.

Immunofluorescence results showed long-term expression and colocalization of AAV8-GFP and KOS/62 in coinfected rabbit TG. IF analysis was performed to demonstrate neuronal colocalization of vector and virus. Sections were incubated with either mouse anti-GFP or mouse anti-β-galactosidase primary antibodies. Alexa Fluor 548 (Invitrogen) or goat anti-mouse Alexa Fluor 488 (Invitrogen) was used as the secondary antibody for immunofluorescent visualization using a Leica deconvolution microscope with Slidebook 5.0. The TG section on the slide was divided into three equal sections (Q1 to Q3 [A to C]) to represent the colocalization of virus and vector over the entire TG cross-section. DAPI (4′,6-diamidino-2-phenylindole) staining (in blue) represents nuclei of satellite cells present in the sections. All images are shown in ×10 magnification.