FIG 9.

Overview of DISC GP85 vaccination schedules and the preconception vaccine study. (a and b) In the initial characterization of the immune response to the DISC vaccine, two vaccine regimens were employed: R1 (a) and R2 (b). DISC virus inoculations are indicated with the arrow along with a virus symbol. Animals were bled for immune response measurements at the indicated weeks post-initial vaccination. Animals were euthanized at the end of the vaccination schedule (and after confirmation of seroconversion) to determine the T cell response via an ELISPOT assay as described in Materials and Methods. (c) Preconception vaccine study (group 1) following vaccine regimen R1. During the late second trimester of pregnancy, animals were challenged with wild-type GPCMV (10⁵ PFU) and then allowed to go to term. (d) Control nonvaccinated animals (group 2) from the congenital vaccine study.