TABLE 1.
Virus replication in KO cell lines
| Cell line | Virus replicationa |
||
|---|---|---|---|
| vD10Rev | vD9muD10mu | vΔE3L | |
| HAP1 | |||
| Controlb | + | − | − |
| PKR KO | + | − | + |
| RNase L KO | + | − | − |
| PKR+RNase L DKO | + | − | + |
| A549 | |||
| Control | + | − | − |
| PKR KO | + | − | − |
| RNase L KO | + | − | − |
| PKR+RNase L DKO | + | +c | + |
| Xrn1 KO | − | − | − |
| Xrn1+PKR+RNase L TKO | + | +c | + |
a
Cells were harvested at 24 h after infection, and virus titrations were performed by plaque assay in permissive BHK-21 cells. The presence (+) or absence (−) of replication is indicated.
b
Infected HAP1 parental cells had undetectable RNase L activity.
c
The increase in virus titer was significant but less than that of vD10rev.