FIG 5.

Antibodies used for leukocyte identification are cell type specific. (A) Gene expression from sorted cell populations. At least 10,000 cells were sorted, total RNA was purified, and genomic DNA was removed. cDNA was synthesized, and residual RNA was removed via RNase H digestion. The cDNA concentration was normalized for each sample, and gene-specific primers were used for RT-PCR. Note that CD8 T cells and B cells were sorted from splenocytes. Due to the low frequency of CD4⁺ T cells in the spleen, sorting was performed using cells from lymph node tissue. (B) Summary of RT-PCR results, organized into distinct leukocyte modules.