FIG 7.

Antigen-specific T cell responses are heterogeneous between individuals and have broad protein specificity across multiple tissue compartments. (A) Viral protein-specific IFN-γ-secreting T cell responses from ferrets (FT) 2466 and 2476 at 10 dpi with A/California/04/09 (H1N1) in the spleen, cLN, and PBMC. T cell responses were not detectable in influenza virus-naive ferret tissues (data not shown). (B) T cell responses from PBMCs of FT 2466 and 2476 as well as FT 3938 (aerosol contact infected, 23 dpi). (C) Antigen-specific T cell reactivity from the PBMCs, represented as a percentage of the total response for each individual tested, depicted as a pie graph (left to right, FT 2466, FT 2476, and FT 3938). pHA-NT, peptides spanning the HA1 domain from pandemic hemagglutinin (subtype H1); pHA-CT, peptides spanning the HA2 domain from pandemic H1; pNA, N1 subtype of neuraminidase from the pandemic H1N1 virus; NP, nucleoprotein; NS1, nonstructural protein 1; M1, matrix 1 protein.