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. 2016 Aug 12;90(17):7980–7990. doi: 10.1128/JVI.00946-16

FIG 4.

FIG 4

Effect of PHA-408 on replication of SC35 and SC35M viruses. (A) MLE-15 cells were preincubated for 1 h with the indicated concentrations of PHA-408 or vehicle. Cells were then stimulated for 20 min with TNF-α and analyzed by immunoblotting for IκBα phosphorylation. (B) MLE-15 cells were preincubated for 1 h with PHA-408 and stimulated for 6 h with TNF. The expression of IL-6 mRNA was quantified by qPCR. Error bars show SEM obtained from two independent experiments performed in triplicate. *, P < 0.05. All other differences had P values of <0.01. (C) MLE-15 cells were treated with 3 μM PHA-408 or vehicle. These cells and MLE-15 NEMO− and MLE-15 p65− cells were infected with SC35 and SC35M (MOI of 0.001). Virus titers were determined 24 h p.i. Error bars show SEM from two independent experiments performed in triplicate. The P values are indicated by asterisks: *, P < 0.05; **, P < 0.01.