FIG 4.
KSHV K1 is required for efficient reactivation from latency. (A and B) A total of 5 × 105 stable iSLK cells latently infected with WT KSHV, KSHV-K1FLAG, KSHV-K15×STOP, KSHV-K1REV, and KSHVΔK1 were plated and examined by fluorescence microscopy in the absence of any induction (A) or after being treated with 1 μg/ml of doxycycline in DMEM supplemented with 2% FBS for 72 h (B). Images were taken at a ×4 magnification. hpi, hours postinfection. (C) Graph depicting the number of GFP-positive cells per field that were quantitated following reactivation.