FIG 4.

Alignment of DhlB proteins from Xanthobacter and Starkeya spp. Note the high sequence divergence of DhlB2 from all the other DhlB proteins. The alignment was generated in ClustalX (58) and then visualized with GeneDoc (59). Shading indicates sequence conservation at that position, as follows: black, 100%; dark gray, 80%; light gray, 60%. Bars below and above the alignment indicate residues known to be essential for catalysis in other haloacid dehalogenases and residues forming the hydrophobic pocket, respectively (60).