FIG 4.

Effect of a glpD deletion on competitive fitness of a Δpcm mutant. Cultures of JV1120 (wild type) and JV1168 (Δpcm mutant) (A), JV1169 (ΔglpD mutant) (B), or JV1170 (ΔglpD Δpcm mutant) (C) were grown individually to stationary phase, mixed at 1:10 (○), 1:1 (△), or 10:1 (□) ratios and allowed to compete for 10 days. Samples were quantitated by viable counts on the indicated days and the relative competitive index (RCI) calculated as described in the text. Closed squares (■) indicate the mean RCI, with error bars representing one standard deviation in each direction. Asterisks indicate the mean RCI being different from an RCI of 0 (dashed line) with P < 0.05.