FIG 6.

Role of (p)ppGpp and polyphosphate in stationary-phase persister formation in isoaspartyl repair-deficient mutants. Persister frequency was determined as described at the onset of stationary phase and 12, 24, and 36 h later for ΔrelA, ΔphoB, and ΔrelA ΔspoT mutants in the following backgrounds: wild type, derivatives of JV1120 (JV1191, JV1192, and JV1199, respectively) (A); ΔglpD Δpcm mutant, derivatives of JV1177 (JV1197, JV1198, and JV1201, respectively) (B); and Δpcm mutant, derivatives of JV1121 (JV1193, JV1194, and JV1200, respectively) (C). Points are averages of the results from three trials, and the error bars represent one standard deviation. Asterisks indicate points significantly (P < 0.05) different from wild type.