TABLE 1.
Bacterial strains, plasmids, and bacteriophages
| Strain, plasmid, or bacteriophage | Comments | Reference or source |
|---|---|---|
| Strains | ||
| Y. enterocolitica | ||
| 6471/76 (YeO3) | Serotype O:3, wild type; human stool isolate | 23 |
| 6471/76-c (YeO3-c) | Virulence plasmid-cured derivative of YeO3 | 23 |
| YeO3-ϕR1-RT-R2 | ϕR1-RT-resistant spontaneous derivative of YeO3 | This work |
| YeO3-ϕR1-RT-R7 | ϕR1-RT-resistant spontaneous derivative of YeO3 | This work |
| YeO3-ϕR1-RT-R9 | ϕR1-RT-resistant spontaneous derivative of YeO3 | This work |
| YeO3-R1 | (=YeO3-c-R1) Spontaneous rough strain | 22 |
| YeO3-hfq::Km | Hfq::Km-GenBlock, Kmr; urease negative | Leskinen et al., submitted for publication |
| YeO3-R1-Cat17 | ompF::Cat-Mu derivative of YeO3-R1 | This work |
| YeO3-R1-Cat17::pSW25T_OmpF | cis-complemented ompF::Cat-Mu strain | This work |
| YeO3-R1-M164 | waaF::Cat-Mu derivative of YeO3-R1, Clmr | 79 |
| YeO3-R1-M196 | galU::Cat-Mu derivative of YeO3-R1, Clmr | 79 |
| YeO3-R1-M205 | hldE::Cat-Mu derivative of YeO3-R1, Clmr | 79 |
| YeO3-c-OC | 25 | |
| YeO3-c-OCR | 25 | |
| K14 | Serotype O:9 | |
| gc815–73 | Serotype O:5,27 | 80 |
| 8081 | Serotype O:8 | 81 |
| Escherichia coli | ||
| BL21 Star (DE3) PLysS | Invitrogen | |
| DH10B | ||
| Plasmids | ||
| pTM100 | 82 | |
| pTM100_OmpF | Complementation plasmid with wild-type ompF gene cloned into pTM100 | This work |
| pSW25T | Suicide vector | 83 |
| pSW25T_OmpF | Complementation suicide plasmid with wild-type ompF gene cloned into pSW25T | This work |
| pCDF Duet-1 | pCloDF13 replicon, T7lac promoter and 2 MCSs, each with an optional N-terminal His6 tag sequence; streptomycin resistance marker | Novagen |
| pET21a(+) | ColE1 (pBR322) replicon, T7lac promoter, N-terminal T7 tag sequence, and optional C-terminal His6 tag sequence; ampicillin resistance marker | Novagen |
| pCDF Duet-1 Gp37 | Phage TG1 ORF250 (bp 4 to 1830) cloned in frame into MCS1 of pCDF Duet-1 for expression of N-terminal His6-tagged protein Gp37 | This study |
| pCDF Duet-1 Gp37-Gp38 | Phage TG1 ORF251 cloned into MCS2 of pCDF Duet-1 Gp37 for coexpression of N-terminal His6-tagged Gp37 and tail fiber assembly chaperone Gp38 | This study |
| pET21a(+) Gp57A | Phage TG1 ORF143 cloned into MCS of pET21a(+) for expression of general trimerization chaperone Gp57A | This study |
| Bacteriophages | ||
| TG1 | This study | |
| ϕR1-RT | This study |