Fig. 3.

fam222bb and fam222ba double mutants display no CCM-specific vascular phenotype. A Schematic of Fam222bb and Fam222ba domain structure, indicating the position of fam222bb^hu10539 and fam222ba^hu10755 amino acid frameshifts and premature stop mutations (red bars). FAM222A: domain of unknown function. Examples of sequencing reads of fam222bb (B) and fam222ba (C) wild-type, heterozygous and homozygous zebrafish embryos; the respective 10-bp deletion is marked in the wild-type read. D Brightfield picture and confocal micrograph of 3 dpf fam222bb/fam222ba double mutant zebrafish trunk bearing Tg(kdrl:egfp)^s843 (green).