Fig. 4.

Spheroid sprouting of HUVECs transfected with negative control siRNA and siRNA against FAM222B. Top row: after transfection, HUVEC spheroids were embedded in a collagen matrix under basal condition and with addition of 25 ng/ml VEGF or FGF2 growth factors. Middle row: transfection of HUVEC with siRNA against FAM222B led to ∼85% mRNA knockdown rates compared to non-silencing control siRNA. Bottom: average sprout length and number of sprouts per spheroid are not significantly altered between control conditions and FAM222B knockdown. n = 4 experiments. Scale bar = 50 µm. Bar graphs depict mean values, error bars show standard error of the mean.