Figure 1.
He‐CAP‐induced cell death in U937 cells. Cells were treated with He‐CAP at a dose of (1, 2, 3 and 4 min.). (A) Cell viability assessment and (B) annexin V‐FITC/PI assay were carried out at 12, 18 and 24 hrs after He‐CAP treatment. (C) Viability of cells in the presence or absence of Pt‐NPs. (D) Cell growth or cell proliferation. Cells were harvested after treatment with He‐CAP in the presence or absence of Pt‐NPs. The number of cells/ml was counted at 24, 48 and 72 hrs to assess proliferation. Data are presented as mean ± SD. *P < 0.05, **P < 0.005. Data shown are representative of five independent experiments. He‐CAP, Helium‐based cold atmospheric plasma.