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. 2016 Jun 2;20(9):1737–1748. doi: 10.1111/jcmm.12880

Figure 2.

Figure 2

Effect of Pt‐NPs on apoptosis induced by He‐CAP in U937 cells. (A) DNA fragmentation assay (B) Percentages of early apoptotic and secondary necrotic cells were analysed 24 hrs after He‐CAP treatment with or without Pt‐NPs by flow cytometry. Data are presented as mean ± SD. *P < 0.05, **P < 0.005. Data shown are representative of five independent experiments. (C) Morphological features of apoptosis in U937 cells following treatment with He‐CAP in the presence or absence of Pt‐NPs; cells were harvested 24 hrs after treatment, signs of apoptotic features in response to He‐CAP were detected by Giemsa staining and then examined under a microscope at ×400 magnification. He‐CAP, Helium‐based cold atmospheric plasma.