Figure 3.

Effect of Pt‐NPs on He‐CAP‐induced ROS formation in U937 cells. U937 cells were treated with He‐CAP for (4 min.) in the presence or absence of Pt‐NPs. The percentage of cells with elevated level of ROS species were analysed by flow cytometry immediately after He‐CAP. (A) DCFH staining, (B) HE staining. Data are presented as the mean ± SD. *P < 0.05, as compared with He‐CAP treatment alone. Data shown are representative of five independent experiments. He‐CAP, Helium‐based cold atmospheric plasma; ROS, reactive oxygen species.