Figure 7.

Effects of Pt‐NPs on He‐CAP‐induced Fas externalization and caspase‐8 activation. (A) Expression of Fas detected by Western blot analysis. Changes in the fraction of U937 cells with Fas externalization were analysed by flow cytometry using an anti‐Fas FITC‐conjugated antibody after treatment with He‐CAP (4 min.) for 18 hrs in the presence or absence of Pt‐NPs. (B) Caspase‐8 activity in U937 cells induced by the He‐CAP treatment with or without Pt‐NPs was also measured with a FLICE/caspase‐8 colorimetric protease kit. Data are presented as mean ± SD. *P < 0.05, **P < 0.005 compared with the He‐CAP alone. Data shown are representative of five independent experiments. He‐CAP, Helium‐based cold atmospheric plasma.