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The levels of 15d‐PGJ2 that were secreted by BMSCs with TNF‐α stimulation in vitro. BMSCs (5 × 105/well) were stimulated with different concentrations of TNF‐α in six‐well plate with non‐serum DMEM medium. The 15d‐PGJ2 levels of supernatant were determined by ELISA after 24 hrs stimulation with TNF‐α. Data were presented as means ± S.E.M. *P < 0.05 versus PBS control group; #P < 0.05 versus TNF‐α 5 ng/ml group; ^P < 0.05 versus TNF‐α 10 ng/ml group.
