Figure 2.
Quantification of protein transduction levels via western blot. (a) GFP and β-actin protein expression was detected utilizing western blotting in the s-EGFP-9R and d-EGFP-9R groups. (b) Quantification of GFP protein transduction levels was performed utilizing the relative intensity. The relative intensity in s-EGFP-9R Group at 24 hours was significantly higher than that at 0 hours and at 24 hours in the s- enhanced green fluorescent protein (EGFP) groups. The EGFP transduction level at 48 hours in the d-EGFP-9R Group was significantly higher compared with that at 0 hours and that at 24 hours in the s-EGFP Group, while EGFP transduction level at 48 hours in the s-EGFP-9R Group indicates no significant change compared with that at 0 hours and that at 24 hours in the s-EGFP group.