Table 2.

Novel miRNAs in P. euphratica.

	TRS	Sequences (5′–3′)	Arm	LM (nt)	MEF (kcal/mol)	LP (nt)	GC%	MEFI
miR-n1	8	gguaguucgaucguggaauu	5p	20	−57.8	244	0.34	0.69
miR-n2	564	gggaauguagccugacucgaga	5p	22	−38.4	84	0.50	0.91
miR-n3		uggugccacgcugcgugcgac	3p	21	−33.7	63	0.59	0.91
miR-n4	18	uuagucuuaauaauuguguga	5p	21	−44	80	0.19	2.93
miR-n5	24	gcaugaggggagucacgcagg	3p	21	−46.6	86	0.55	0.99
miR-n6		ucauuugagcaagaaauauu	3p	20	−52.2	129	0.34	1.19
miR-n7		gcggcagcaucaagauucaca	5p	21	−46.8	109	0.48	0.90
miR-n8		caggcggucuccuuggcua	5p	19	−78.9	215	0.47	0.78
miR-n9		cagaauugcagugccuugauc	3p	21	−41.4	98	0.40	1.06
miR-n10		gaaugcugaccgaauauggau	5p	21	−27.5	70	0.41	0.95
miR-n11		ucaauggccauuguaagagag	5p	21	−23.4	81	0.40	0.73
miR-n12		ucggaccaggcuucauccccc	3p	21	−30.4	84	0.50	0.72
miR-n13		guuccucugagcacaucacug	5p	21	−30.9	80	0.46	0.84
miR-n14		aagauggagaagcagggcacg	5p	21	−62.2	154	0.46	0.88
miR-n15	233	ugaugacugaucuugagcaug	5p	21	−41.1	81	0.49	1.03
miR-n16		gugggcuugugauccaagu	5p	19	−94.9	287	0.46	0.71
miR-n17		cuuggcuauuguagauaaccc	3p	21	−39.5	93	0.46	0.92
miR-n18	122	ggauugucgucugguucgaug	5p	21	−33.2	83	0.41	0.98
miR-n19		ucagcgcugcauucaaucaug	3p	21	−39.7	104	0.42	0.90
miR-n20	5	cucaagaaagccgugggaga	3p	20	−42.3	127	0.42	0.80
miR-n21		uaucccuuuggauuccuccuu	3p	21	−43.8	82	0.51	1.04
miR-n22		guuccuccuagcuucuucagug	5p	22	−61	207	0.40	0.74
miR-n23		guuccucugagcacuucaacg	5p	21	−33.1	80	0.46	0.89
miR-n24	7	cucccccucaagggcuuccugu	5p	22	−54.9	110	0.51	0.98
miR-n25	14	ucggaccaggcuucauuccuc	3p	21	−25.7	82	0.46	0.68
miR-n26	53	aguuuguucguggaacugaug	5p	21	−28.6	80	0.48	0.75
miR-n27	14	ucgagaucuguucaauaguac	5p	21	−89.9	248	0.33	1.11
miR-n28	6	uauuggccugguucacucaga	5p	21	−36.4	106	0.40	0.87
miR-n29	385	aggugcaggugcuagugcagg	3p	21	−51.3	151	0.39	0.87
miR-n30	564	uaaugcugucugguucgagac	5p	21	−35.3	80	0.50	0.88
miR-n31	160	uugacagaagagagugagcac	5p	21	−42	85	0.38	1.31
miR-n32	14	cucggaccaggcuucauuccc	3p	21	−36.9	73	0.52	0.97
miR-n33	1	uuucgggaagugaaauuugga	3p	21	−35.8	113	0.36	0.87
miR-n34	26	ggucaugcucugacagccucacu	3p	23	−31	89	0.45	0.78
miR-n35	2	ugagaucuuuugaauuauaucauu	5p	24	−58.9	233	0.28	0.89
miR-n36	74	auugaugguagaauuacuugg	3p	21	−32	78	0.28	1.45
miR-n37	102	auaaagugggcaguagagagu	5p	21	−48.3	78	0.40	1.56
miR-n38	26	caacccuuccagauauuggug	3p	21	−47.3	61	0.52	1.48
miR-n39	3	uggacugaagggagcuccuuc	3p	21	−79.2	184	0.43	0.99
miR-n40	108	uucauguaucucucgacucuu	3p	21	−39.9	93	0.34	1.25
miR-n41	130	auuugcuuguauuuaacuccc	5p	21	−51.1	75	0.43	1.60
miR-n42	1	ugacaggcucuucucucucaug	3p	22	−64.8	172	0.41	0.93
miR-n43	1	gaauguugucuggcucgagga	5p	21	−42.6	110	0.41	0.95
miR-n44	15	auaugcguuuuugucccucgc	3p	21	−83.8	264	0.42	0.75
miR-n45		cacgugcuccccuucuccaac	3p	21	−34.9	66	0.55	0.97
miR-n46		caugugcucuagcucuccagc	3p	21	−41.7	74	0.47	1.19
miR-n47	57	uauuauaaccaagacuaaugc	5p	21	−37.7	120	0.24	1.30
miR-n48	2709	uuacacuggcaacucauuuga	3p	21	−62.6	186	0.37	0.92
miR-n49	38	cgggguuggguguucuaugag	3p	21	−42.3	85	0.49	1.01
miR-n50	1	uuuaugcguuuuuggcccucg	3p	21	−36.7	119	0.39	0.80
miR-n51	331	acacacacuggagaagggaag	3p	21	−47.2	71	0.56	1.18
miR-n52	1	aaguaccugauagcaacucaagu	5p	23	−84.4	278	0.46	0.66
miR-n53	1	gaagauuuggugugccucugaggu	3p	24	−68.6	226	0.39	0.78
miR-n54		ugccaaaggagaguugcccug	3p	21	−40.2	87	0.49	0.93
miR-n55	53	uuguaaugauauagaugugau	5p	21	−43.2	103	0.26	1.60
miR-n56	4	ucaauggagcccagauguaga	5p	21	−33.4	78	0.36	1.19
miR-n57	2	uugaauaugcuugagcugugu	5p	21	−36	64	0.36	1.57
miR-n58	1	ugaaaagacgaaagaguagaga	5p	22	−63.2	140	0.44	1.02
miR-n59	1	aggggcaaaaaucgcauaaga	5p	21	−43.9	105	0.43	0.98
miR-n60	3	uuaagucgcaugcaugauuga	3p	21	−68.9	234	0.38	0.77
miR-n61	1	uguugaugaugauaaauu	5p	18	−64.3	292	0.21	1.04
miR-n62	1	uagccaaggaugacuugcccgc	5p	22	−50.7	122	0.44	0.94
miR-n63	21	uuauaaagccauaagaagucc	5p	21	−47.3	76	0.26	2.37
miR-n64	11	aagagacucuuguauguauguaug	5p	24	−41.1	59	0.31	2.28
miR-n65	795	uucugucgccggaaagauggug	5p	22	−43.7	82	0.48	1.12
miR-n66	4	agaaagaagacagaaggcagcgug	5p	24	−67.8	105	0.44	1.47
miR-n67	13	acauaaauugcaaggaacuua	5p	21	−30.2	72	0.31	1.37
miR-n68		gugaggcugguuucacagagc	3p	21	−41.6	115	0.50	0.72
miR-n69	90	cucgaugaccgaucuugggca	5p	21	−38.3	84	0.49	0.93
miR-n70		guuccccugagcacuucacu	5p	20	−42.1	82	0.44	1.17
miR-n71		auuagauguagguuugauacgugu	3p	24	−31	166	0.23	0.79
miR-n72	6	uuucaguuugauucagcaugc	5p	21	−49.7	193	0.36	0.72
miR-n73		auaaacuugauuauguggaauaua	5p	24	−127	254	0.25	2.02
miR-n74	183	ugaacucucucccucaacugc	5p	21	−41.5	85	0.48	1.01
miR-n75	10	uuuguaauuuguaaacuuguu	3p	21	−73.3	123	0.29	2.04
miR-n76		ugaggccuuugggggagaguga	3p	22	−40.7	89	0.53	0.87
miR-n77		gcgaccccaggucaggcgga	5p	20	−98.2	220	0.64	0.70
miR-n78	1	uuuuucuugguguuguuggacucu	3p	24	−70.1	148	0.41	1.17
miR-n79		uaagaucauugccuauuggagacu	3p	24	−29.2	81	0.37	0.97
miR-n80		ugcucacuucucuucugucagc	3p	22	−49.2	85	0.46	1.26
miR-n81		uugacagaagauagagagcacu	5p	22	−41.2	87	0.41	1.14
miR-n82		uuuggaaggaagauuugaagu	3p	21	−61.9	180	0.42	0.81
miR-n83		auuuaaauugauucugaaacuauu	3p	24	−88.9	221	0.17	2.34
miR-n84		uuggacugaagggagcucccuc	3p	22	−80.4	179	0.46	0.97
miR-n85		aggugcuggugccggugcagg	3p	21	−40.3	112	0.40	0.90
miR-n86		ccucgcucccagcugacaccc	5p	21	−73.7	195	0.49	0.77
miR-n87		aaauugaugaauuuauggagu	3p	21	−47.2	155	0.40	0.76
miR-n88	24	gugcucacgucucuucugucag	3p	22	−53.3	85	0.47	1.33
miR-n89	4010	uuccaaagggaucgcauugauc	5p	22	−42.5	93	0.37	1.25
miR-n90		uaugggaggauuggacaggac	5p	21	−34.1	75	0.48	0.95
miR-n91		uagccaaggacgauuugccugu	5p	22	−47.7	99	0.53	0.92
miR-n92	93	aaccccuaguugcacguggacgug	5p	24	−49.6	109	0.59	0.78
miR-n93		uucgaucugggucaaaucuuuc	3p	22	−30.4	56	0.52	1.05
miR-n94		ggagcgaccuggaaucacaug	5p	21	−38.3	76	0.61	0.83

TSR, total read count of miRNA^*; nt, nucleotide; LM, length of miRNA; LP, length of precursors.