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. 2016 May 30;18(2):221–228. doi: 10.22074/cellj.2016.4317

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Fig.1 — Increased level of peroxisome proliferator-activated receptor γ (PPARγ) expression during spontaneous cardiac differentiation of mouse embryonic stem cells (mESCs). A. Illustrated protocol of mESCs during cardiac differentiation: early embryoid bodies (EBs, day 2) were cultured in fetal calf serum (FCS) enriched media for additional 5 days. Emerged cardiac progenitor cells (CPCs) were allowed to differentiate within 8 days and B. Quantitative real-time polymerase chain reaction (qPCR) analysis of PPARγ expression level of mESCs (stem cell), CPCs (day 7) and beating bodies (day 15). Relative expression level of PPARγ was quantified and normalized with β-tubulin V. Represented value bars are the mean of triplicate independent experiments ± SEM. *; Indicates a significant difference between the treated and control groups at day 7 and day 15 (P<0.05).