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. 2016 May 30;18(2):221–228. doi: 10.22074/cellj.2016.4317

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Fig.4 — Modulation of peroxisome proliferator-activated receptor γ (PPARγ) activity on cardiac cell and smooth muscle cell markers. A. Quantitative real-time polymerase chain reaction (qPCR) analysis of α-Cardiac actin and α-MHC (cardiac cell markers) in the beating bodies, B. qPCR quantitative analysis of SMAα and SM22α (smooth muscle cell markers) in the beating bodies. The relative expression level of target genes were quantified and normalized with β-tubulin V. *; Indicates a significant difference between the treated and control groups (P<0.05), C-E. Morphological illustration of the generated beating bodies. The reduced size of beating bodies was observed in the GW9662 (GW)-treated samples and F-H. Immunostaining of the generated beating bodies derived from the treated cardiac precursor cells and the control group against α-Cardiac actin on day 15 of the experiment (scale bar; 200 μm).