Table 1.
Genes | Sequencing primer (5´-3´) | Annealing temp (˚C) | Accession no. |
Mesp1 | F:CAGTCCCTCATCTCCGCTCT | 62 | NM_008588.2 |
R: CAGTCCCTCATCTCCGCTCT | |||
Nkx2.5 | F:TTAGGAGAAGGGCGATGAC | 57 | NM_008700.2 |
R: AGGGTGGGTGTGAAATCTG | |||
Mef2c | F:CGAGTGTAAGTGTCTAATG | 54 | NM_001170537.1 |
R: CCTATTGTCAGAATTGCTAT | |||
α-Cardiac actin | F:GTGTGACGACGAGGAGAC | 61 | NM_009608.3 |
R: GTGTGACGACGAGGAGAC | |||
α-MHC | F:CAGAAGCCTCGCAATGTC | 58 | NM_001164171.1 |
R: CGGTATCAGCAGAAGCATAG | |||
SMAα | F:TCAGGGAGTAATGGTTGGAATG | 61 | NM_007392.2 |
R: TTGGTGATGATGCCGTGTTC | |||
SM22α | F:TGTCACTCCTGTTAGCATTCC | 54 | NM_011526.5 |
R: GGTCACTCTTCTTCTCCATAGC | |||
Gapdh | F:TGCCGCCTGGAGAAACC | 58 | NM_008084.2 |
R: TGAAGTCGCAGGAGACAACC | |||
PPARγ | F:TGAGACCAACAGCCTGAC | 60 | NM_001127330.1 |
R: GTTCACCGCTTCTTTCAAATC | |||