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. 2016 May 30;18(2):221–228. doi: 10.22074/cellj.2016.4317

Table 1.

Primers used for gene expression analysis by quantitative real-time polymerase chain reaction


Genes Sequencing primer (5´-3´) Annealing temp (˚C) Accession no.

Mesp1 F:CAGTCCCTCATCTCCGCTCT 62 NM_008588.2
R: CAGTCCCTCATCTCCGCTCT
Nkx2.5 F:TTAGGAGAAGGGCGATGAC 57 NM_008700.2
R: AGGGTGGGTGTGAAATCTG
Mef2c F:CGAGTGTAAGTGTCTAATG 54 NM_001170537.1
R: CCTATTGTCAGAATTGCTAT
α-Cardiac actin F:GTGTGACGACGAGGAGAC 61 NM_009608.3
R: GTGTGACGACGAGGAGAC
α-MHC F:CAGAAGCCTCGCAATGTC 58 NM_001164171.1
R: CGGTATCAGCAGAAGCATAG
SMAα F:TCAGGGAGTAATGGTTGGAATG 61 NM_007392.2
R: TTGGTGATGATGCCGTGTTC
SM22α F:TGTCACTCCTGTTAGCATTCC 54 NM_011526.5
R: GGTCACTCTTCTTCTCCATAGC
Gapdh F:TGCCGCCTGGAGAAACC 58 NM_008084.2
R: TGAAGTCGCAGGAGACAACC
PPARγ F:TGAGACCAACAGCCTGAC 60 NM_001127330.1
R: GTTCACCGCTTCTTTCAAATC