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. 2016 May 19;9(4):e1191715. doi: 10.1080/19420889.2016.1191715

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© 2016 The Author(s). Published with license by Taylor & Francis.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.

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Figure 2. — Pre-incubation of PC12 cells with EVs derived from H₂O₂-treated cells results in ∼2-fold higher levels of pTyr23AnxA2 upon subsequent treatment with H₂O₂, as compared to cells exposed to H₂O₂ only. PC12 cells were untreated (A), treated for 15 min with 1 mM H₂O₂ only (B), or treated for 15 min with 1 mM H₂O₂ after their pre-incubation for 2 h with EVs released from cells exposed for 1 h to H₂O₂ (C). pTyr23AnxA2 (green, A-C) was detected using monoclonal antibodies. The DAPI-stained nuclei are shown in blue. Scale bars: 10 μm. The diagrams below each panel show the corresponding intensity profiles along the lines indicated in the respective panels. Panel 2D: Corrected total cell fluorescence (CTCF) was measured from cells (20–30) in the samples shown (panels A–C); CTCF were then divided by the number of cells measured.