Table 1.
Pharmacological properties of wild‐type α1β3γ2S and mutant GABAA receptors
| Subunit composition | EC50 (μM) | n H | n |
|---|---|---|---|
| α1β3γ2S | 61.9 ± 2.1 | 1.44 ± 0.03 | 7 |
| α1I227Aβ3γ2S | 24.3 ± 1.4 *** | 1.18 ± 0.04 | 5 |
| α1L231Aβ3γ2S | 89.1 ± 6.6 ** | 1.32 ± 0.07 | 5 |
| α1M235Aβ3γ2S | 19.4 ± 2.2 *** | 1.25 ± 0.13 | 6 |
| α1M235Wβ3γ2S | 2.9 ± 0.4 *** | 0.79 ± 0.06 | 6 |
| α1L268Aβ3γ2S | 15.7 ± 1.9 *** | 1.19 ± 0.1 | 5 |
| α1β3T262Aγ2S | 116.7 ± 9.1 *** | 1.41 ± 0.06 | 5 |
| α1β3T262Sγ2S | 6.4 ± 0.1 *** | 1.33 ± 0.14 | 6 |
| α1β3N265Sγ2S | 57.2 ± 4.5 | 1.23 ± 0.09 | 6 |
| α1β3T266Aγ2S | 143.7 ± 14.0 *** | 1.21 ± 0.08 | 6 |
| α1β3R269Aγ2S | 108.4 ± 5.9 *** | 1.51 ± 0.05 | 5 |
| α1β3M286Aγ2S | 138.4 ± 9.2 *** | 1.33 ± 0.08 | 5 |
| α1β3M286Wγ2S | 7.1 ± 0.3 *** | 1.33 ± 0.14 | 7 |
| α1β3F289Sγ2S | 7.0 ± 1.2 *** | 0.90 ± 0.08 | 7 |
EC50 concentrations (μM) and Hill coefficients (n H) are given for each receptor as mean ± SEM for n number of cells tested. Statistical significance of difference from wild‐type was calculated using a one‐way ANOVA followed by a Dunnett's mean comparison test. ** P < 0.01; *** P < 0.001.