Fig. 1.

SMYD3 is upregulated during colorectal tumorigenesis (A) SMYD3 mRNA expression increases during transition from normal to tumor colon mucosa in APC^Min/+ mice. Tissue samples were obtained from control APC^Min/+ mice (n = 10) and APC^Min/+ mice treated with 12 mg/kg of azoxymethane (AOM) (n = 10). (B) Colorectal adenomas and carcinomas of APC^Min/+ mice overexpress SMYD3 protein and show increased global levels of H4K5me and H3K4me2. cMYC and pERK expression was assessed as an internal control of the experiment. (C) SMYD3 expression profile correlates with mRNA expression of its target genes. β-actin was used as a loading control for immunoblotting and for real-time PCR data normalization. Statistical analysis was performed using Student’s t-tail test; *P < 0.05 was considered statistically significant.