Fig. 2.

SMYD3 shows high levels of expression in CRC cell lines. SMYD3 protein (A, C) and mRNA (B, D) expression is enhanced in several human CRC cell lines. (A, C) SMYD3 protein expression correlates with the global level of targeted histone methyl marks [H3K4me2 and H4K5me] and (B, D) with the transcriptional activation of its target genes in the indicated cell lines. The levels of a SMYD3 non-targeted methyl mark [H3K27me3] are not modulated. In B and D, fold induction is normalized to mRNA levels observed in NCM460 cells (arbitrarily set as 1). β-actin was used as a loading control for immunoblotting and for real-time PCR data normalization. Statistical analysis was performed using Student’s t-tail test; *P < 0.05 was considered statistically significant.