Fig. 3.

SMYD3 is required for proper cancer cell growth. In HT29 (A, B, C) and HCT116 (D, E, F) CRC cell lines, SMYD3 inhibition by RNAi impairs cell proliferation (A, D), decreases the level of targeted histone methyl marks [H4K5me, H3K4me2] and ERK 1/2 activation (B, E), and reduces the expression level of SMYD3 target genes (C, F). A non-targeted methyl mark [H3K27me3] was not affected by RNAi-mediated SMYD3 ablation. HT29 and HCT116 CRC cell lines were transfected with control siRNAs or SMYD3-specific siRNAs for 48 h. Cell proliferation was calculated using the WST-1 assay. β-tubulin was used as a loading control for immunoblotting and β-actin was used for real-time PCR data normalization. Statistical analysis was performed using Student’s t-tail test; *P < 0.05, **P < 0.01, and ***P < 0.001 were considered statistically significant.